Composite

Part:BBa_K1809032:Design

Designed by: David Morgan   Group: iGEM15_WPI-Worcester   (2015-08-26)


Const. Promoter-RBS-BclA-MpAFP-DT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 397
    Illegal AgeI site found at 487
    Illegal AgeI site found at 778
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 749


Design Notes

Spliced together a cell surface expression tag and the antifreeze protein to test cell surface activity.


Source

Composite of parts BBa_K1423005 and BBa_K1809031 without the YFP tag.

References